山东治疗白癜风最好的医院 https://m-mip.39.net/nk/mipso_4663696.htmlEffectsofOPonMyocardialInfarctSizeandNoReflowinaRatMyocardialIschemia/ReperfusionModelCardiovasc?Drugs?TherOriginalArticleEarlyRecent,Feb08,./s---7本文由“天纳”临床学术信息人工智能系统自动翻译点击文末“阅读原文”下载本文PDFPurposeThepresentstudywastodeterminewhetherOPcouldlimitmyocardialinfarctionsizeandtheno-reflowphenomenoninaratmyocardialischemia/reperfusionmodel.本研究旨在探讨OP对大鼠心肌缺血再灌注模型心肌梗死面积及无再流现象的影响。MethodsRatheart–isolatedmitochondria(RHM)wereusedtoinvestigatemitochondrialrespirationandmitochondrialreactiveoxygenspecies(mtROS)generationbothinnormalconditionsandinischemia/reperfusion-mimickingconditions(usinghighconcentrationsofsuccinate).Humanskeletalmusclemyoblasts(HSMM)inculturewereusedtoinvestigatethecellularintermittentdeprivationinenergysubstratesandoxygenasreportedinischemia/reperfusionconditions.Invivo,ratswereanesthetizedandsubjectedto30?minofleftcoronaryarteryocclusionfollowedby3?hofreperfusion.RatswererandomizedtoreceiveOPasanintravenousinfusionstartingeither5?minpriortocoronaryarteryocclusion(preventive),or5?minpriortoreperfusion(curative),ortoreceivevehiclestarting5?minpriortocoronaryarteryocclusion.Infusionscontinueduntiltheendofthestudy(3?hofreperfusion).用大鼠心脏离体线粒体（RHM）研究正常和模拟缺血/再灌注条件下（使用高浓度琥珀酸）线粒体呼吸和线粒体活性氧（mtROS）的产生。以体外培养的人骨骼肌成肌细胞（HSMM）为研究对象，研究了缺血/再灌注条件下细胞能量底物和氧的间歇性剥夺。在体内，大鼠被麻醉，左冠状动脉闭塞30min，再灌注3h。将大鼠随机分为两组，一组在冠状动脉闭塞前5分钟（预防性）或再灌注前5分钟（治疗性）开始静脉输注OP，另一组在冠状动脉闭塞前5分钟开始静脉输注载体。持续输注直至研究结束（再灌注3小时）。ResultsRHMtreatedwithOPshowedaconcentration-dependentreductionofsuccinate-inducedmtROSgeneration.InHSMMcells,OPtreatment(5–10?μM)during48Hpreventedthereductioninthesteady-statelevelofATPmeasuredjustafterreperfusioninjuriesanddecreasedthemitochondrialaffinitytooxygen.Invivo,myocardialinfarctsize,expressedasthepercentageoftheischemicriskzone,wassignificantlylowerintheOP-treatedpreventivegroup(44.5?±?2.9%)versusthatinthevehiclegroup(57.0?±?3.6%;p??0.05),withanon-significanttrendtowardasmallerinfarctsizeinthecurativegroup(50.8?±?3.9%).TheareaofnoreflowasapercentageoftheriskzonewassignificantlysmallerinboththeOP-treatedpreventive(28.8?±?2.4%;p?=?0.vsvehicle)andcurativegroups(30.1?±?2.3%;p?=?0.04vsvehicle)